There are a few ways to do this: In particular, you should avoid exporting data using the Bio-Formats Exporter because the files it writes might not be readable later by release versions of Bio-Formats or other OME-compliant tools. It can open many dozens of proprietary life sciences formats, and standardize their acquisition metadata into a common OME data model. Page Discussion View source History. Furthermore, let's say you need to manually outline different regions and have the counts described above reported separately per region.
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For a more detailed description of each plugin, see the Bio-Formats page of the ImageJ wiki. Fiji ships release versions of Bio-Formats. You can also configure any specific options for each format—for example, for QuickTime, you can toggle between Apple's QTJava library or Bio-Formats's built-in support.
Create account Log imagem. To report a bug in Bio-Formats, please see reporting a bug in Bio-Formats.
Bio-Formats
You can also perform more targeted metadata queries using the Bio-Formats macro extensions. See especially the Using Bio-Formats page for detailed instructions.

If you have problems with this on a computer installed by your IT department, you may need to have them update your permissions. The Autoscale button is optional.
You can also upgrade the Bio-Formats plugin directly from ImageJ. I do this with the macros at http: It is likely to be less robust than working with files on disk, so when possible we recommend downloading your data to disk and using the regular Bio-Formats Importer instead. It will also extract and set basic metadata values such as spatial calibration if they are available in the file.
Page Discussion View source History. Note that you cannot use the bifoormat option because Bio-Formats does not work in headless mode, even when running as a macro. There may be p,ugin bugs. See Bio-Formats Downloads page.
You can see a list of supported file formats on the Formats tab, toggle each format on or off which is useful, for example, if your file is being detected as the wrong pulginand toggle whether each format bypasses the importer options dialog using the "Windowless" checkbox.
Issue with ROI importation using the Bio-format plugin
The Bio-Formats Macro Extensions plugin prints out the available commands to the ImageJ log window, along with instructions for using them. It is often difficult to determine for sure that this is the problem - the only error message that pretty much guarantees it is a NoSuchMethodException.
Bio-Formats is fully scriptable in a macro, and callable from a plugin. The Bio-Formats plugins come with a set of macro extensions to enable additional functionality from macros.

The version of ImageJ running on my personal computer opens files from Zeiss Zen and Nikon Elements as they displayed bioofrmat when you choose Hyperstack and Composite in the menu. The Bio-Formats Windowless Importer is a version of the Bio-Formats Importer plugin that runs with the last used settings to avoid popping up any additional dialogs beyond the file chooser.
Most need customization for individual datasets. Skip to contentSkip to search. Click here for a webpage primer on working with channels. In addition, you can see a list of plugib helper libraries used by Bio-Formats on the Libraries tab. ImageJ is an image processing and analysis application written in Java, widely used in the life sciences fields, with an extensible plugin infrastructure.
Other plugins you may find useful are at http: Get it at fiji.
Bio-Formats - ImageJ
Bioforma with Bio-Formats plugin opens raw data exactly as they appear in the original imaagej with the benefit of preserving the underlying raw data.
There are more recent versions of Bio-Formats that work with more formats, but this works for all the Zen and Elements files I've encountered at work. Want to know more about the history of ImageJ and how it is pioneering in the field of image analysis, way ahead of commercial products?

More details on installation here:
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